UJI TOKSISITAS DAN AKTIVITAS ANTIKANKER TERHADAP  SEL KANKER PAYUDARA MCF-7 DARI FRAKSI  KAYU KUNING  (Arcangelisia flava (L) Merr).

Nanda Nanda; Yanda  Rahmasari; Miming  Andika; Nola  Rahmadasmi; Oryza Sativa  Fitriani

doi:10.51225/jps.v9i2.113

Authors

Nanda Nanda Universitas Fort de Kock
Yanda Rahmasari Universitas Fort de Kock
Miming Andika Universitas Fort de Kock
Nola Rahmadasmi Universitas Fort de Kock
Oryza Sativa Fitriani Universitas Fort de Kock

DOI:

https://doi.org/10.51225/jps.v9i2.113

Keywords:

kayu kuning, anti-cancer, BSLT, MCF-7

Abstract

Arcangelisia flava (L.) Merr. or kayu kuning is a medicinal plant that has long been used traditionally In Southeast Asia, it is reported to have potential anticancer activity. This study aims to assess the toxicity. Profile and cytotoxic activity of the ethanol extract and its fractions. Toxicity was evaluated using the Brine Shrimp Lethality Test (BSLT), and cytotoxic activity against MCF-7 breast cancer cells was assessed with the WST-8 assay. Phytochemical screening results showed the presence of alkaloids, flavonoids, tannins, and steroids in the ethanol extract and the fractions obtained. The LC₅₀ values of the ethanol extract, ethanol fraction, ethyl acetate fraction, and n-hexane fraction were 624.43 ppm, 552.96 ppm, 950.86 ppm, and 1095.62 ppm, respectively. Based on toxicity criteria (LC₅₀ < 1000 ppm), the ethanol extract, ethanol fraction, and ethyl acetate fraction were classified as having moderate toxicity. The ethanol fraction showed the lowest LC₅₀ value and was therefore selected for further testing on MCF-7 cells. Cytotoxicity testing showed that the ethanol fraction had an IC₅₀ value of 189.86 ppm, indicating moderate cytotoxic activity. These findings indicate that the ethanol fraction of A. flava has the potential to be a source of candidate anticancer compounds and warrants further research through the isolation and characterization of its active compounds..

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