EKSPLORASI AKTIVITAS ANTIBAKTERI ACTINOMYCETES YANG BERASOSIASI DENGAN SPONS LAUT PULAU SAMALONA
DOI:
https://doi.org/10.51225/jps.v10i1.158Keywords:
Actinomycetes, antibacterial, marine sponge, Escherichia coli, StreptomycesAbstract
The increasing prevalence of antibiotic resistance has intensified the need to discover novel antibacterial compounds from marine microorganisms. This study aimed to investigate the antibacterial potential of actinomycetes associated with marine sponges collected from Samalona Island, Indonesia. Sponge-associated actinomycetes were isolated using selective media and screened for antagonistic activity against Staphylococcus aureus and Escherichia coli. The most active isolate was subsequently fermented for 21 days, and its secondary metabolites were extracted using ethyl acetate and methanol. Antibacterial activity was evaluated using the agar diffusion method. A total of 18 actinomycete isolates were successfully obtained, among which isolate SP-6 exhibited antibacterial activity against both test organisms. The ethyl acetate extract at a concentration of 20% (w/v) demonstrated the strongest antibacterial activity, producing inhibition zones of 8.32 mm against S. aureus and 8.54 mm against E. coli. The production of antibacterial metabolites reached its maximum on the 18th day of fermentation before gradually declining. Morphological characterization indicated that isolate SP-6 possessed characteristics consistent with the genus Streptomyces. These findings suggest that marine sponge-associated actinomycetes from Samalona Island represent a promising source of novel antibacterial compounds with potential applications in future pharmaceutical development.
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